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cd4+cd25+ regulatory t cell isolation kit, mouse  (Miltenyi Biotec)


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    Miltenyi Biotec cd4+cd25+ regulatory t cell isolation kit, mouse
    Cd4+Cd25+ Regulatory T Cell Isolation Kit, Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 231 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd25+cell+isolation+kit/CD4%2BCD25%2B+Regulatory+T+Cell+Isolation+Kit%2C+mouse/custom%40130-091-041%4042480527
    Average 96 stars, based on 231 article reviews
    cd4+cd25+ regulatory t cell isolation kit, mouse - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Isolation:

    Article Title: Human CD4 + effector T lymphocytes generated upon TCR engagement with self-peptides respond defectively to IL-7 in their transition to memory cells
    Article Snippet: Central (CCR7 + , T CM ) and effector memory T cells (CCR7 − , T EM ) 24 were isolated from CD4 + CD45RA − lymphocytes by FACS using an anti-CCR7 antibody (3D12; BD Biosciences, Bedford, MA, USA). .. CD25 − Tmem were isolated by negative selection using the CD25 + cell isolation kit (Miltenyi Biotec). ..

    Selection:

    Article Title: Human CD4 + effector T lymphocytes generated upon TCR engagement with self-peptides respond defectively to IL-7 in their transition to memory cells
    Article Snippet: Central (CCR7 + , T CM ) and effector memory T cells (CCR7 − , T EM ) 24 were isolated from CD4 + CD45RA − lymphocytes by FACS using an anti-CCR7 antibody (3D12; BD Biosciences, Bedford, MA, USA). .. CD25 − Tmem were isolated by negative selection using the CD25 + cell isolation kit (Miltenyi Biotec). ..

    Cell Isolation:

    Article Title: Human CD4 + effector T lymphocytes generated upon TCR engagement with self-peptides respond defectively to IL-7 in their transition to memory cells
    Article Snippet: Central (CCR7 + , T CM ) and effector memory T cells (CCR7 − , T EM ) 24 were isolated from CD4 + CD45RA − lymphocytes by FACS using an anti-CCR7 antibody (3D12; BD Biosciences, Bedford, MA, USA). .. CD25 − Tmem were isolated by negative selection using the CD25 + cell isolation kit (Miltenyi Biotec). ..

    Article Title: CD25+CD4+ cells contribute to Th2 polarization during helminth infection by suppressing Th1 response development.
    Article Snippet: DC were isolated, as previously described (17), by incubating splenocytes for 15 min with CD11c-specific microbeads (Miltenyi Biotec, Auburn, CA), followed by passage through a MACS magnet, or by using AutoMACS (Miltenyi Biotec). .. CD25 and CD25 cells from the CD11c fraction or from a negatively selected CD4 cell fraction were MACS-sorted using a CD25 cell isolation kit (Miltenyi). .. In some experiments CD4 or B cells were depleted using purified mAbs GK1.5 or RA3.3AG, respectively, followed by sheep antirat Ig Dynabeads (Dynal Biotech, Lake Success, NY) and magnetic sorting.

    Magnetic Cell Separation:

    Article Title: CD25+CD4+ cells contribute to Th2 polarization during helminth infection by suppressing Th1 response development.
    Article Snippet: DC were isolated, as previously described (17), by incubating splenocytes for 15 min with CD11c-specific microbeads (Miltenyi Biotec, Auburn, CA), followed by passage through a MACS magnet, or by using AutoMACS (Miltenyi Biotec). .. CD25 and CD25 cells from the CD11c fraction or from a negatively selected CD4 cell fraction were MACS-sorted using a CD25 cell isolation kit (Miltenyi). .. In some experiments CD4 or B cells were depleted using purified mAbs GK1.5 or RA3.3AG, respectively, followed by sheep antirat Ig Dynabeads (Dynal Biotech, Lake Success, NY) and magnetic sorting.



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    Miltenyi Biotec pan t cells
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    Miltenyi Biotec cat 130 091 041
    <t>CD4</t> + <t>CD25+</t> regulatory T cells isolated from mouse spleen were cocultured with LLC cells: control (sh-NC), CFB-knockdown (sh-CFB), CFB-knockdown with control siRNA (sh-CFB + control siRNA), and CFB/FOS double-knockdown (sh-CFB + FOS siRNA). A ELISA measurement of tryptophan and kynurenine levels in coculture media. B Flow cytometry analysis of Foxp3+ Treg frequency after coculture. The proportion of FOXP3+ cells represents the frequency within the total mixed population of tumor cells and Tregs. C Quantitative PCR analysis of immunosuppressive markers (Foxp3, CTLA4, LAG3, GITR) in Tregs. D Western blot analysis of cytoplasmic and nuclear AhR fractions in Tregs after coculture (raw data in Fig. ). E XRE/AhR luciferase reporter assay in Tregs after coculture. N = 3 experiments. Statistics in A , B , C , E : one-way ANOVA followed by Tukey’s post hoc test. Error bands=Mean and SD.
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    Image Search Results


    CD4 + CD25+ regulatory T cells isolated from mouse spleen were cocultured with LLC cells: control (sh-NC), CFB-knockdown (sh-CFB), CFB-knockdown with control siRNA (sh-CFB + control siRNA), and CFB/FOS double-knockdown (sh-CFB + FOS siRNA). A ELISA measurement of tryptophan and kynurenine levels in coculture media. B Flow cytometry analysis of Foxp3+ Treg frequency after coculture. The proportion of FOXP3+ cells represents the frequency within the total mixed population of tumor cells and Tregs. C Quantitative PCR analysis of immunosuppressive markers (Foxp3, CTLA4, LAG3, GITR) in Tregs. D Western blot analysis of cytoplasmic and nuclear AhR fractions in Tregs after coculture (raw data in Fig. ). E XRE/AhR luciferase reporter assay in Tregs after coculture. N = 3 experiments. Statistics in A , B , C , E : one-way ANOVA followed by Tukey’s post hoc test. Error bands=Mean and SD.

    Journal: Communications Medicine

    Article Title: Complement factor B deficiency promotes lung cancer progression via FOS-mediated tryptophan metabolism and immune suppression

    doi: 10.1038/s43856-026-01648-w

    Figure Lengend Snippet: CD4 + CD25+ regulatory T cells isolated from mouse spleen were cocultured with LLC cells: control (sh-NC), CFB-knockdown (sh-CFB), CFB-knockdown with control siRNA (sh-CFB + control siRNA), and CFB/FOS double-knockdown (sh-CFB + FOS siRNA). A ELISA measurement of tryptophan and kynurenine levels in coculture media. B Flow cytometry analysis of Foxp3+ Treg frequency after coculture. The proportion of FOXP3+ cells represents the frequency within the total mixed population of tumor cells and Tregs. C Quantitative PCR analysis of immunosuppressive markers (Foxp3, CTLA4, LAG3, GITR) in Tregs. D Western blot analysis of cytoplasmic and nuclear AhR fractions in Tregs after coculture (raw data in Fig. ). E XRE/AhR luciferase reporter assay in Tregs after coculture. N = 3 experiments. Statistics in A , B , C , E : one-way ANOVA followed by Tukey’s post hoc test. Error bands=Mean and SD.

    Article Snippet: CD4+CD25+ regulatory T cells and CD8+ T cells were isolated from spleens of 6–8 week-old C57BL/6 mice using the CD4+CD25+ Regulatory T Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany; catalog #130-091-041) and CD8+ T Cell Isolation Kit (Miltenyi Biotec, catalog #130-104-075), respectively.

    Techniques: Isolation, Control, Knockdown, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Real-time Polymerase Chain Reaction, Western Blot, Luciferase, Reporter Assay